Table 1

‚ÄÉMethods of gene transfer

MethodAdvantagesDisadvantages
Herpes simplex virusBroad infectivityCytotoxic, low titres, and short term episomal expression
AdenovirusHigh titres, infects non-dividing cellsLife threatening immunogenecity. Short term episomal expression only
Adenoassociated virusBroad infectivity, infects non- dividing cells, non-cytopathicShort term expression with limited integration
LentivirusPermanently infects non-dividing cells. High viral titresSafety concerns owing to HIV derivation
RetrovirusPermanent integration, well characterisedPoor infection efficiency. Does not infect non-dividing cells
LiposomesAvoids use of cytopathic virusLow efficiency of transfection of cells