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Effects of ABCA1 gene polymorphisms on risk factors, susceptibility and severity of coronary artery disease
  1. Zhan Lu1,
  2. Zhi Luo1,
  3. Aimei Jia2,
  4. Irfan Muhammad1,
  5. Wei Zeng1,
  6. Azhe Shiganmo3,
  7. Xueli Chen4,
  8. Yongyan Song5
  1. 1Department of Cardiology, Affiliated Hospital of North Sichuan Medical College, Nanchong, Sichuan, China
  2. 2School of Preclinical Medicine, North Sichuan Medical College, Nanchong, Sichuan, China
  3. 3School of Medical Imaging, North Sichuan Medical College, Nanchong, Sichuan, China
  4. 4Department of Anaesthesiology, North Sichuan Medical College, Nanchong, Sichuan, China
  5. 5Scool of Preclinical Medicine, and Nanchong Key Laboratory of Metabolic Drugs and Biological Products, North Sichuan Medical College, Nanchong, China
  1. Correspondence to Dr Yongyan Song, Biochemistry, North Sichuan Medical College, Nanchong, China; songyongyan2014{at}foxmail.com

Abstract

Background The relationships between the rs1800976, rs4149313 and rs2230806 polymorphisms in ATP binding cassette protein A1 and severity of coronary artery disease (CAD) remain unclear.

Methods Four hundred and forty-two patients with CAD and 217 CAD-free subjects were enrolled in this study. The rs1800976, rs4149313 and rs2230806 polymorphisms were genotyped by PCR-RFLP. Severity of CAD was evaluated by Gensini score system, number of stenotic coronary vessels and extent of coronary stenosis.

Results C allele of the rs1800976 polymorphism, G allele of the rs4149313 polymorphism and A allele of the rs2230806 polymorphism were found to be risk alleles for CAD (p<0.05 for all). In patients with CAD, C allele of the rs1800976 polymorphism was associated with high levels of hypersensitive C reactive protein (hs-CRP) and cystatin c (CysC), and its frequency increased with percentiles of Gensini score, number of stenotic coronary vessels and extent of coronary stenosis (p<0.05 for all). The subjects with GA genotype of the rs4149313 polymorphism had higher levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C), apolipoprotein B and hs-CRP than those with AA genotype (p<0.05 for all). The subjects with AA genotype of the rs2230806 polymorphism had higher levels of TC, LDL-C and uric acid than those with GA genotype (p<0.05 for all). No associations between the rs4149313 or rs2230806 polymorphism and severity of CAD were detected.

Conclusions The rs1800976 polymorphism is significantly associated with the occurrence and severity of CAD, which is possibly mediated by hs-CRP and CysC.

  • ABCA1
  • polymorphism
  • coronary artery disease
  • severity
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Footnotes

  • ZL and ZL are joint first authors.

  • ZL and ZL contributed equally.

  • Contributors YYS, ZLu and ZLuo conceived of the study, participated in the design and drafted the manuscript. ZLuo, AMJ and IM extracted DNA and genotyped the three polymorphisms. AS, XLC and WZ collected the clinical data. YYS and ZLuo performed the statistical analyses. All authors reviewed and approved the final manuscript.

  • Funding This project was supported by the Key Project of Education Department of Sichuan Province, People's Republic of China (17ZA0172) and the Cooperative Project on Scientific Research between Nanchong city and North Sichuan Medical College, People's Republic of China (NSMC20170403).

  • Competing interests None declared.

  • Patient consent for publication Not required.

  • Ethics approval The study protocol was reviewed and approved by the Ethics Committee of North Sichuan Medical College (No. 2015012).

  • Provenance and peer review Not commissioned; externally peer reviewed.

  • Data availability statement All data relevant to the study are included in the article or uploaded as supplementary information.

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