Purpose To explore the association of adiponectin (AD) and adiponectin receptor (ADR) gene single-nucleotide polymorphisms (SNPs) with genetic susceptibility to rheumatoid arthritis (RA) in a Chinese population.
Study design Five AD SNPs (rs266729, rs2241766, rs1063537, rs2082940 and rs1063539) and two ADR SNPs (rs7539542 and rs12342) were genotyped in a cohort of 617 patients with RA and 639 healthy controls. Seven SNPs were genotyped using TaqMan genotyping assays on the Fluidigm 192.24 system. The concentration of AD in plasma was examined by ELISA.
Results Patients with RA showed a considerably lower plasma level of AD than healthy controls (p=0.002). No significant differences were observed for the distribution of allele and genotype frequencies of rs266729, rs2241766, rs2082940, rs1063539, rs7539542 and rs12342 SNPs between patients with RA and controls. The genotype effects of recessive and dominant models were also analysed, but no marked evidence for association was found. However, further analysis in female patients with RA showed that the frequency of the AD gene rs1063539 GG genotype was nominally significantly higher in patients who were anti-cyclic citrullinated peptide (anti-CCP) antibody-positive (p=0.040). No significant differences in serum AD level were observed in patients with RA with different genotypes.
Conclusions rs266729, rs2241766, rs2082940 and rs1063539 in the AD gene and rs7539542 and rs12342 in the ADR gene are possibly not associated with genetic susceptibility to RA, but the A D gene rs1063539 locus was possibly associated with anti-CCP in RA female patients.
- adiponectin receptor
- single-nucleotide polymorphisms
- rheumatoid arthritis
- autoimmune disease
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Contributors Y-LZ, T-PZ, JW, B-ZL, X-ML, H-FP and D-QY planned the study. Y-LZ, T-PZ and JW collected samples and performed the experiments. Y-LZ, T-PZ and B-ZL conducted statistical ananlyses. Y-LZ, T-PZ, X-ML, H-FP and D-QY clarified the results. Y-LZ and T-PZ drafted the manuscript. All authors read and approved the final manuscript.
Funding This work was supported by grants from the National Natural Science Foundation of China (81473058).
Competing interests None declared.
Patient consent for publication Not required.
Ethics approval The study was reviewed and approved by the medical ethics committee of Anhui Medical University (20150115). All participants were included after obtaining informed consent.
Provenance and peer review Not commissioned; externally peer reviewed.
Data availability statement All data relevant to the study are included in the article or uploaded as supplementary information.
Author note Yu-Lan Zhao and Tian-Ping Zhang contributed equally to this work and should be considered co-first authors.
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