ORIGINAL ARTICLE

Effects of topical treatment of sodium butyrate and 5-aminosalicylic acid on expression of trefoil factor 3, interleukin 1ß, and nuclear factor B in trinitrobenzene sulphonic acid induced colitis in rats

M Song, B Xia and J Li

Departments of Internal Medicine and Geriatrics, Zhongnan Hospital; Research Centre of Digestive Diseases; Key Laboratory of Allergy and Immune related Diseases, Wuhan University School of Medicine, Wuhan, the People’s Republic of China

Correspondence to:
Correspondence to:
Professor B Xia
Department of Internal Medicine and Geriatrics, Wuhan University Zhongnan Hospital, Donghu Road 169, Wuhan 430071, Hubei Province, People’s Republic of China; bingxia2004{at}yahoo.com.cn

Background and Aims: Butyrate enemas have been shown to be effective in treatment of ulcerative colitis, but the mechanism of the effects of butyrate is not totally known. This study evaluates effects of topical treatment of sodium butyrate (NaB) and 5-aminosalicylic acid (5-ASA) on the expression of trefoil factor 3 (TFF3), interleukin 1ß (IL1ß), and nuclear factor B (NFB) in trinitrobenzene sulphonic acid (TNBS) induced colitis in rats.

Methods: Distal colitis was induced in male Wistar rats by colonic administration of TNBS and colonically treated with NaB, 5-ASA, combination of NaB and 5-ASA, and normal saline for 14 consecutive days. Colonic damage score, tissue myeloperoxidase (MPO) activity, TFF3 mRNA expression, serum IL1ß production, and tissue NFB expression were determined, respectively.

Results: Treatment of NaB, 5-ASA, and the combination improved diarrhoea, colonic damage score, and MPO activities, increased TFF3 mRNA expression, and decreased serum IL1ß production and tissue NFB expression. The combination therapy of NaB and 5-ASA had better effects than any other single treatment.

Conclusions: The combination of topical treatment of NaB and 5-ASA was effective for relieving and repairing colonic inflammation and the effects were related to stimulation of TFF3 mRNA expression and down-regulation of IL1ß production and NFB expression.

Abbreviations: IBD, inflammatory bowel disease; SCFA, short chain fatty acid; TFF3, trefoil factor 3; NaB, sodium butyrate; IL1ß, interleukin 1ß; NFB, nuclear factor B; 5-ASA, 5-aminosalicylic acid; MPO, myeloperoxidase; TNBS, trinitrobenzene sulphonic acid

Keywords: sodium butyrate; 5-aminosalicylic acid; trefoil factor 3; interleukin 1; nuclear factor B

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