ORIGINAL ARTICLE

Polymorphisms of CD14 gene and TLR4 gene are not associated with ulcerative colitis in Chinese patients

Q S Guo¹, B Xia¹, Y Jiang¹, S A Morré², L Cheng¹, J Li¹, J B A Crusius² and A S Peña²

¹ Department of Internal Medicine and Geriatrics, Research Centre of Digestive Diseases of Zhongnan Hospital; Laboratory of Inflammation and Autoimmune Diseases of the Institute of Allergy and Immune Related Diseases, Wuhan University Medical School, Wuhan, PR of China
² Laboratory of Immunogenetics, VU University Medical Centre, Amsterdam, Netherlands

Correspondence to:
Correspondence to:
Professor B Xia
Department of Internal Medicine and Geriatrics, Wuhan University Zhongnan Hospital, No 169 Donghu Road, Wuhan 430071, Hubei Province, PR of China; bingxia2004{at}yahoo.com.cn

Background and aims: Toll-like receptor 4 and CD14 are the components of the lipopolysaccharide receptor complex. The aim of this study was to investigate the associations between polymorphisms TLR4 Asp299Gly and CD14 C-260T and Chinese patients with ulcerative colitis (UC).

Methods: Using a polymerase chain reaction based restriction fragment length polymorphism, the study genotyped polymorphisms TLR4 Asp299Gly and CD14 C-260T in 114 patients with UC and 160 healthy controls in the Chinese Han population. Moreover a comparison was made with 170 healthy Dutch white subjects.

Results: No TLR4 Asp299Gly mutation was detected in any patients or healthy controls in the Chinese Han population, which was similar to Japanese subjects, but the mutation occurred in 10% of the Dutch white subjects. There were no significant differences of CD14 genotypes between healthy controls and the patients with UC.

Abbreviations: UC, ulcerative colitis; CD, Crohn’s disease; IBD, inflammatory bowel disease; TLR4, toll-like receptor 4; LBP, LPS binding protein

Keywords: gene polymorphism; toll-like receptor-4; CD14; ulcerative colitis

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